| Catalogue Number | Description | Pack Size | Price |
|---|---|---|---|
| P4-0201 | AAV Purification Midi Kit – 63300 | 4-8 Preps | £435.00 |
P4-0201
AAV Purification Midi Kit – 63300
Norgen’s AAV purification kit provides a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a precipitation and centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1 mL).
- AAV Purification from any input – cell fraction, media fraction, or mixed cells and media
- Rapid purification within 2 to 2.5 hours
- High AAV recovery, up to 90%
- No specialized equipment needed
- Purification from a variety of AAV serotypes
- Purify AAV from 8 mL to 45 mL input
- Yields highly active AAV for in vitro experiments
- Up to 50X sample concentration
- Multiple purifications (8 per kit) can be done in parallel. More parallel purifications can be done with additional kits
Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV purification kit provides a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a precipitation and centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1 mL).
Specification
| Specification Column Binding Capacity; At least 1 x 1010 AAV particles as determined by qPCR AAV Vector Serotype;Any Average Recovery; > 80% Input Type; Cells, media, or mixed Input Volume; 8 mL - 45 mL Minimum Elution Volume; 1 mL Time to Complete 4 Purifications; 2 - 2.5 hours Storage Conditions and Product Stability DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. All the reagents should remain stable for at least 1 year in their unopened containers. |
Documentation
| Literature P4-0201 Literature 63300 |
| Protocol P4-0201 Protocol 63300 |
| MSDS P4-0201 MSDS 63300 |
