| Catalogue Number | Description | Pack Size | Price |
|---|---|---|---|
| P4-0432 | AAV Purification Maxi Slurry Kit – 63250 | 1-10 Preps | £1,285.00 |
P4-0432
AAV Purification Maxi Slurry Kit – 63250
Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV purification kits provide a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a precipitation and centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion and column purification. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1-10 mL) using the optional concentration step.
- AAV Purification from any input – cell fraction, media fraction, or mixed cells and media
- Rapid purification within 2.5 to 3.5 hours, with optional concentration step
- 1-10 mL final elution volume
- High AAV recovery, up to 90%
- No specialized equipment needed
- Purification from a variety of AAV serotypes
- Purify AAV from 90 mL to 900 mL of input per run
- Combine 2 kits for 1,800 mL input purification per run
- Yields highly active AAV for in vitro experiments
- Up to 200-fold sample concentration
- Multiple purifications (10 per kit, 90 mL each) can be done in parallel, or 1 single large purification of 900 mL of input
Specification
| Specification Column Binding Capacity; At least 1 x 1010 AAV particles as determined by qPCR AAV Vector Serotype; Any Average Recovery; 50 - 80% Input Type; Cells, media, or mixed Input Volume; 90 mL - 900 mL Minimum Elution Volume; 1 mL - 10 mL Time to Complete Purifications; 2.5 - 3.5 hours Storage Conditions and Product Stability DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. All the reagents should remain stable for at least 1 year in their unopened containers. |
Documentation
| Protocol P4-0432 Protocol 63250 |
