HRPL Lunimol-Based Western Blotting Kit

  • HPLHorseradish Peroxidase Visualisation System
  • Safer, contains no DMSO
  • Reduced background
  • Longer shelf life, enhanced stability

The HRPL Kit causes light to be produced when any molecule labeled with horseradish peroxidase (HRP) is exposed to the reagents in the HRPL Kit. This light can be recorded on X-ray film or counted in a tube or microtiter plate luminometer. The light output peaks approximately 2 minutes after introduction of the reagent, and lasts for more than 1 hour.

An exceptional property unique to National Diagnostics' HRPL Kit is its broad dynamic range. Samples as small as 2 picograms can be readily detected. Because larger samples do not overexpose film, however, samples as large as 2 mg can be quantified within the same assay. As an alternative to the colored dye visualization methods, the HRPL Kit offers the advantages of greater sensitivity without exposure to hazardous dye reagents or DMSO.

National Diagnostics' HRPL Kit contains 120 ml of HRPL Substrate A and 120 ml of HRPL Substrate B. Sufficient reagents are provided to process 3000 cm2 of membrane.

Storage: All system components are best stored at 4oC and are stable for 1 year

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